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NanoDrop_Spectrophotometer

The Expression Analysis Facility has a Nanodrop ND-1000 Spectrophotometer available for use during normal working hours. This device will perform full-spectrum UV-Vis absorbance analyses (220-750nm), and is designated for use in the facility for measuring absorbance of DNA, RNA, microarray labeling dyes, and proteins (while the nanodrop can read cell culture ODs and protein assays utilizing binding dyes, such as the Bradford assay, our instrument is not to be used for those types of readings).

Advantages of the Nanodrop include:

* 1 ul only of sample required
* Large dynamic range: 2-3700 ng/ul of dsDNA (manufacturer’s specs, this is probably pushing it at both ends)
* No dilutions for most samples
* No cuvettes or capillaries
* 10 second measurement